FIG 5.

Pseudomonas isolate LPH1 corrinoid biosynthesis gene is required for support of amoebal growth. (A) Pseudomonas isolate LPH1 (Ps) cobalamin biosynthesis genes using Pseudomonas denitrificans nomenclature with predicted riboswitches indicated. cobC*, cbiG*, and cbiW* refer to homologs of Salmonella enterica. For clarity, ps4553 (flanked by a predicted riboswitch and cobL) is not depicted. (B to D) Rescue of Pseudomonas isolate LPH1 cobalamin synthase deletion mutant (ps2662::aacC1, ΔcobV) for supporting amoebal growth on S. elongatus by genetic complementation (B) and supplementation with cyanocobalamin (Cbl) (C), with corresponding CFU from assay wells (D). Gray indicates the same growth curve controls. (E) Amoebal growth curves with S. elongatus, with the addition of supplemented cyanocobalamin (+Cbl) and fixed Pseudomonas isolate LPH1. Values are averages of triplicate wells plus or minus SEM.