TABLE 3.

Substrate specificity of MLG1

Substrate	Glycosidic bond	Relative activity (%)a
Laminarin	β-1,3-1,6	100
Pachyman	β-1,3-1,6	40.2
Cardran	β-1,3	32.0
Lentinan	β-1,3-1,6	NDb
Barley glucan	β-1,3-1,4	29.2
Lichenan	β-1,3-1,4	ND
Cellulose	β-1,4	ND
Pustulan	β-1,6	ND

^aReleased reducing sugar after incubation of MLG1 and each substrate was matured by the PAHBAH (4-hydroxybenzoic acid hydrazide) method. Relative activity of each substrate was indicated as a ratio (%) of absorbance of each substrate toward that of laminarin.

^bND, not detected.