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. 2017 May 1;83(10):e00233-17. doi: 10.1128/AEM.00233-17

FIG 1.

FIG 1

Colony PCR (cPCR) results confirm the deletion of the pta and buk genes. (A) cPCR results using primers YW1044 and YW1045 flanking the upstream and downstream sequences of the homologous recombination region of the pta gene in C. saccharoperbutylacetonicum deltpta (lane 1, 2,278 bp) and the wild type (lane 2, 3,280 bp). (B) cPCR results using primers YW953 and YW954 flanking the upstream and downstream sequences of the homologous recombination region of the buk gene in C. saccharoperbutylacetonicum deltbuk (lane 3, 2,061 bp) and the wild type (lane 4, 3,119 bp). (C) cPCR results using primers YW953 and YW954 flanking the upstream and downstream sequences of the homologous recombination region of the buk gene in C. saccharoperbutylacetonicum deltptabuk (lane 5, 2,061 bp) and the C. saccharoperbutylacetonicum deltpta strain (lane 6, 3,119 bp). The NEB 1-kb DNA ladder was used as the marker (lane M), with numbers on the left representing the band length in kb.