TABLE 1.
Plasmid used for transformation | Promotera | Generation for: |
No. of mutants/no. of colonies screened (mutation rate, %) | |
---|---|---|---|---|
Mutant band starting to be detected from TGYLE liquid culture using cPCR | Obtainment of mutant strain | |||
pYW34-deltpta | PsRNA | Second | Fourth | 3/16 (18.75) |
pSHW1-deltpta | Pvegb | Second | Second | 2/16 (12.5) |
pSHW2-deltpta | Pvegc | Second | Seventh | 1/16 (6.25) |
pSHW3-deltpta | PJ23119 | Second | Second | 12/16 (75) |
PsRNA, small RNA promoter from C. beijerinckii; Pvegb, veg promoter from B. subtilis; Pvegc, hypothetical veg gene promoter from C. saccharoperbutylacetonicum; PJ23119, J23119 promoter from E. coli.