. 2017 May 1;83(10):e00233-17. doi: 10.1128/AEM.00233-17

TABLE 1.

Comparison of genome editing efficiencies using CRISPR-Cas9 with different promoters for gRNA expression

Plasmid used for transformation	Promoter^a	Generation for:		No. of mutants/no. of colonies screened (mutation rate, %)
Plasmid used for transformation	Promoter^a	Mutant band starting to be detected from TGYLE liquid culture using cPCR	Obtainment of mutant strain	No. of mutants/no. of colonies screened (mutation rate, %)
pYW34-deltpta	P_sRNA	Second	Fourth	3/16 (18.75)
pSHW1-deltpta	P_vegb	Second	Second	2/16 (12.5)
pSHW2-deltpta	P_vegc	Second	Seventh	1/16 (6.25)
pSHW3-deltpta	P_J23119	Second	Second	12/16 (75)

P_sRNA, small RNA promoter from C. beijerinckii; P_vegb, veg promoter from B. subtilis; P_vegc, hypothetical veg gene promoter from C. saccharoperbutylacetonicum; P_J23119, J23119 promoter from E. coli.