FIG 2.

SDS-PAGE of IEF-purified tailocin BceTMilo. Protein subunits of tailocin BceTMilo were dissociated as described in Materials and Methods and loaded on a (A) 10 to 20% Tris-HCl SDS gel or (B) 7.5% Tris-HCl SDS gel. Lane 1, molecular mass standards (Precision Plus protein standard from Bio-Rad; 10 to 250 kDa); lane 2, subunits of tailocin BceTMilo. Proteins were visualized using Coomassie blue staining. Please note that the white lines between lanes 1 and 2 for both panel A and panel B indicate that a lane was removed from each of the original gels, since the data were not presented in Results.