FIG 4.

Effect of Burkholderia LPS on BceTMilo. Increasing amounts of purified LPS from (A) B. cenocepacia strain PC184 (sensitive) and (B) B. cenocepacia strain AU10487 (resistant) were mixed with tailocin BceTMilo and allowed to incubate for 30 min. A 1:2 dilution series (see Materials and Methods) of mixtures from the experiments represented in panel A and panel B were spotted onto overlays seeded with strain PC184 to determine residual BceTMilo activity. Results are representative of triplicate experiments.