TABLE 1.
Tailocin BceTMilo efficiency of killing
| Tail major protein | Protein mass (ng/10 μl) | No. of protein moleculesa (1014 per ml) | No. of tailocin particlesb (1011 per ml) | No. of KUc (1011 per ml) | EOKd |
|---|---|---|---|---|---|
| Sheath (43 kDa) | 91 | 1.3 | 6.27 | 3.89 | 0.6 |
| 144 | 2.0 | 9.89 | 3.89 | 0.4 | |
| 151 | 2.1 | 10.40 | 12.30 | 1.2 | |
| 473 | 6.6 | 32.40 | 12.30 | 0.4 | |
| Tube (17 kDa) | 36 | 1.3 | 6.20 | 3.89 | 0.6 |
| 94 | 3.3 | 16.30 | 3.89 | 0.2 | |
| 322 | 11.0 | 55.80 | 12.30 | 0.2 | |
| Avg | 0.5 |
a
Number of protein molecules = [(protein mass × 1E−9) × 100/molecular mass] × Avogadro's number.
b
Number of tailocin particles = number of protein molecules/204 (calculated as the number of protein molecules [tube or sheath]/the number of tailocin particles).
c
KU, killing units.
d
EOK = number of KU/number of tailocin particles.