TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| Burkholderia cenocepacia | ||
| Burkholderia cenocepacia BC0425 | Producer of BceTMilo, ET12 epidemic lineage, clinical isolate | Laboratory collection |
| Burkholderia cenocepacia AU10487 | Clinical isolate | Laboratory collection |
| Burkholderia cenocepacia K56-2 | ET12 epidemic lineage, O-antigen+ | Sokol et al. (46) |
| Burkholderia cenocepacia J2315 | ET12 epidemic lineage, O-antigen− | Laboratory collection |
| Burkholderia cenocepacia PC184 | Midwest epidemic lineage, O-antigen− | Laboratory collection |
| Burkholderia cenocepacia BC0425 ΔrecA | BC0425, deleted recA gene | This study |
| Burkholderia cenocepacia K56-2 Δ waaL | K56-2, deleted waaL gene, O-antigen-l-Rham− | This study |
| Burkholderia cenocepacia K56-2Δ waaC | K56-2, deleted waaC gene, O-antigen-l-Rham− Glu− | This study |
| Escherichia coli E. cloni 5-alpha | fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Lucigen |
| Pseudomonas aeruginosa PAO1 | LPS A+ LPS B+, R2 pyocin producer | J. S. Lam (Burrows et al. [47]) |
| Pseudomonas aeruginosa PAO1wbpM | LPS A+ LPS B− | J. S. Lam (Burrows et al. [47]) |
| Pseudomonas aeruginosa PAO1wbpL | LPS A− LPS B− | J. S. Lam (Rocchetta et al. [48]) |
| Pseudomonas aeruginosa PAO1rmlC | LPS A− LPS B− l-Rham− | J. S. Lam (Rahim et al. [49]) |
| Pseudomonas aeruginosa PAO1algC | LPS A− LPS B− l-Rham− Glu− | J. S. Lam (Jarrell et al. [50]) |
| Pseudomonas aeruginosa NIH-K | R1 pyocin producer | ATCC 25350 |
| Pseudomonas aeruginosa NIH-1 | R5 pyocin producer | ATCC 25313 |
| Plasmids | ||
| pRK2013 | Tra+ Mob+ ColE1 replicon Kmr | Laboratory Stock |
| pMo130 | Suicide vector for allelic exchange in Burkholderia; ColE1 ori RK2 oriT xylE sacB Kmr | M. I. Voskuil (51) |
| pMo168 | Replicative vector for Burkholderia; oripBBR1 mob+ xylE Kmr | M. I. Voskuil (51) |
| pMo130::recA-UD | pM0130 with recA upstream and downstream fragments | This study |
| pMo130::waaL-UD | pM0130 with waaL upstream and downstream fragments | This study |
| pmo130::waaC-UD | pM0130 with waaC upstream and downstream fragments | This study |
| pMo168::recA-comp | recA gene cloned into pMo168 | This study |
| pMo168::waaL-comp | waaL gene cloned into pMo168 | This study |
| pMo168::waaC-comp | waaC gene cloned into pMo168 | This study |
a
Kmr, resistance to kanamycin; Rham, rhamnose; Glu, glucose.