TABLE 3.
Kinetic constants for binding RSV-A2 G proteina
| Strain and epitope | Antibody | ka (s−1 · M−1 [104]) | kd (s−1 [105]) | KD (pM) |
|---|---|---|---|---|
| RSV-A2 | ||||
| A | AT42 | 79.8 ± 3.3 | 65.0 ± 13.2 | 809 ± 132 |
| B | AT34 | 31.3 ± 4.6 | 0.4 ± 0.2 | 12.8 ± 6.9 |
| B | AT40 | 59.4 ± 12.4 | 5.8 ± 0.4 | 106 ± 29 |
| B | AT64G | 35.7 ± 6.2 | 0.5 ± 0.2 | 14.7 ± 2.8 |
| B | 131-2G | 28.5 ± 2.0 | 103 ± 2 | 3,640 ± 320 |
| C | AT50 | 21.8 ± 2.5 | 35.1 ± 7.7 | 1,610 ± 340 |
| C | AT51 | —b | — | — |
| C | AT61G | 18.9 ± 4.0 | 200 ± 40 | 10,800 ± 3,000 |
| D | AT32 | 42.5 ± 8.3 | 18.2 ± 2.3 | 459 ± 122 |
| D | AT33 | 73.5 ± 5.2 | 0.8 ± 0.4 | 11.1 ± 4.8 |
| RSV-B | ||||
| A | AT42 | 22.2 ± 1.2 | 4.6 ± 0.5 | 208 ± 33 |
| B | AT34 | 3.76 ± 0.42 | 2.7 ± 0.7 | 719 ± 174 |
| B | AT40 | 5.99 ± 0.50 | 2.3 ± 0.4 | 379 ± 45 |
| B | AT64G | 6.33 ± 0.99 | 4.8 ± 1.1 | 776 ± 169 |
| B | 131-2G | 111 ± 5 | 159 ± 20 | 1,420 ± 120 |
| C | AT50 | — | — | — |
| C | AT51 | — | — | — |
| C | AT61G | — | — | — |
| D | AT32 | — | — | — |
| D | AT33 | — | — | — |
a
Data represent means ± standard deviations from at least two independent measurements. —, no binding detected.
b
AT51 did not bind in this setup (the immobilized antibody is inactive).