TABLE 4.
CDN, ADCC, and ADCPa
| Epitope | Clone | Strain(s) specificity |
KD (pM) for G protein from: |
IC50 (ng/ml) for RSV A/B CDN | % neutralization of HAE cells in culture at 0.2 μg/ml | EC50 (ng/ml) |
||
|---|---|---|---|---|---|---|---|---|
| RSV-A | RSV-B | ADCC | ADCP | |||||
| A | AT42 | A, B | 809 ± 132 | 208 ± 33 | 90.3/11.1 | 69 ± 7 | — | 609 |
| B | AT34 | A, B | 12.8 ± 6.9 | 719 ± 174 | 158/37.2 | 1 ± 0 | 1,352 | 304 |
| B | AT40 | A, B | 106 ± 29 | 379 ± 45 | 30.6/15.1 | 7 ± 2 | 456 | 336 |
| B | AT64G | A, B | 14.7 ± 2.8 | 776 ± 169 | 109/39.9 | — | ND | ND |
| B | 131-2G | A, B | 3,640 ± 320 | 1,420 ± 120 | —/— | 92 ± 11 | ND | ND |
| C | AT50 | A | 1,610 ± 340 | — | —/— | 92 ± 4 | 791 | 798 |
| C | AT51 | A | — | — | —/— | 68 ± 13 | — | — |
| C | AT61G | A | 10,800± 3,000 | — | —/— | — | ND | ND |
| D | AT32 | A | 459 ± 122 | — | 14.7/— | 23 ± 33 | 3,866 | 265 |
| D | AT33 | A | 11.1 ± 4.8 | — | 249/— | 41 ± 21 | — | 279 |
| AM22 | A, B | — | — | 2.6/41.1 | 99 ± 2 | 222 | 119 | |
a
Neutralization and opsonization experiments were performed with antibodies purified from B cell supernatants. Data represent means ± standard deviations from at least three independent measurements. IC50, 50% inhibitory concentration, the concentration of antibody which inhibits 50% of RSV plaque formation in vitro; EC50; 50% effective concentration, the concentration of antibody which results in 50% of neutrophils with phagocytized FITC-labeled RSV-A2-infected HEp-2 cells or 50% ADCC; —, no binding to RSV G protein was detected in the SPR experiment or <50% neutralization, ADCC, or phagocytosis was measured; ND, not determined; AM22, antibody specific for the RSV prefusion F protein.