FIG 5.

IFA of BHK cells infected with VACV. BHK cells were infected with vYLDV-97 (A to C) or vΔA6 (D and E) at an MOI of 0.5 PFU/cell. After 8 h, the infected cells were fixed, permeabilized, and stained with MAbs for A13 (11F7) (29), A11 (10G11) (18), or the V5 epitope, followed by DAPI and goat anti-mouse IgG coupled to Cy3. vYLDV-97 but not vΔA6 also expressed GFP. The fluorescence signal from DAPI is shown in white in the first column. Note the difference in A11 localization between panels A and D. F, viral factory.