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Plaque morphology and growth curves of A6 mutant VACVs. (A) BS-C-1 cells in 12-well plates were infected with the indicated VACVs in semisolid medium for 48 h. The cells were then stained with crystal violet to reveal plaques. (B) BS-C-1 cells in 12-well plates were infected with the indicated viruses at an MOI of 0.5 PFU/cell. Viral titers at 0, 24, and 48 hpi were determined by a plaque assay on HeLa-iA6 cells.
