TABLE 4.

Sulfolobus and E. coli strains used

Strain	Description/genotype	Reference or source
S. solfataricus P1	S. solfataricus isolate with complete genome sequence	DSM 1616 (39, 40)
S. solfataricus P2	S. solfataricus isolate with complete genome sequence	DSM 1617 (41, 39)
S. solfataricus S441	S. solfataricus isolate, SSV1 host	38
S. solfataricus GΘ	S. solfataricus MT4 derivative	42
E. coli EC100D pir+	F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ− rpsL (StrR) nupG pir+(DHFR)	Epicentre, Inc.
E. coli EC100D pir-116	F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ− rpsL (StrR) nupG pir-116(DHFR)	Epicentre, Inc.
E. coli NovaBlue	endA1 hsdR17 (rK12− mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proA+B+ lacIqZΔM15::Tn10] (TetR)	Millipore, Inc.