FIG 1.
PrPCWD amyloid seeding activity is not detected at 24 and 72 h following oronasal exposure. (A) RT-QuIC analysis of tissues collected 24 h postoronasal exposure. Rare tissues, such as the prescapular LN, mandibular LN, and RAMALT, displayed minor SHrPrP substrate conversion; however, rates of amyloid formation were not statistically different from those of corresponding negative-control tissues. Data from two deer (963 and 965) are displayed as means and standard errors of the means (SEM) from 8 replicates, representing 2 separate experiments. LN, lymph node; Retro LN, retropharyngeal lymph node; RAMALT, rectoanal mucosa-associated lymphoid tissue; VNO, vomeronasal organ. (B) RT-QuIC analysis of tissues collected 72 h postmucosal exposure. A few tissues, such as the prescapular LN and spleen, displayed minor SHrPrP substrate amyloid conversion; however, rates of amyloid formation were not statistically different from those of the corresponding negative-control tissues (P > 0.05, unpaired t test). Data from two deer (967 and 968) are displayed as means and SEM from 8 replicates, representing 2 separate experiments. (C) RT-QuIC analysis of negative-control tissues collected 72 h postmucosal exposure. Rare tissues displayed minimal spontaneous amyloid formation of SHrPrP substrate. Data from one deer are displayed as means and SEM from a minimum of 8 replicates and 2 separate experiments.