TABLE 1.
Summary of PrPCWD detection in 96GG WTD by RT-QuIC and TSA-IHC following mucosal exposurea
| Tissue | Value atb: |
|||||||
|---|---|---|---|---|---|---|---|---|
| 1 MPE |
2 MPE |
3 MPE |
4 MPE |
|||||
| QuIC | IHC | QuIC | IHC | QuIC | IHC | QuIC | IHC | |
| Retro LN | 2/3 | 0/3 | 3/3 | 2/3 | 2/2 | 2/2 | 3/3 | 3/3 |
| Tonsil | 0/3 | 2/3 | 2/3 | 3/3 | 2/2 | 2/2 | 3/3 | 3/3 |
| Mandibular LN | 0/3 | 0/3 | 2/3 | 0/3 | 2/2 | 2/2 | 3/3 | 3/3 |
| Parotid LN | 1/3 | 1/3 | 1/3 | 0/3 | 1/2 | 1/2 | 3/3 | 2/3 |
| Prescapular LN | 0/3 | 0/3 | 0/3 | 0/3 | 2/2 | 1/2 | 3/3 | 2/3 |
| Mesenteric LN | 1/3 | 0/3 | 0/3 | 0/1 | 1/2 | 0/2 | 3/3 | 2/3 |
| Hepatogastric LN | 0/3 | 0/3 | 0/3 | 0/3 | 2/2 | 2/2 | 3/3 | 3/3 |
| Ileocecocolic LN | 0/3 | 0/2 | 0/3 | 0/3 | 2/2 | 1/2 | 3/3 | 3/3 |
| Spleen | 0/3 | 0/2 | 0/3 | 0/2 | 2/2 | 1/2 | 3/3 | 2/3 |
| RAMALT | 0/3 | 0/3 | 0/3 | 0/2 | 2/2 | 0/2 | 2/3 | 2/3 |
| VNO | 0/3 | NA | 0/3 | NA | 2/2 | NA | 2/3 | NA |
| Ileum | NA | 0/2 | NA | 0/2 | NA | 1/2 | NA | 2/2 |
| Obex | 0/3 | 0/3 | 0/3 | 0/3 | 0/2 | 0/2 | 0/3 | 0/3 |
a
RT-QuIC and TSA-IHC detected similar patterns of PrPCWD accumulation during early infection: initial detection in oropharyngeal lymph nodes prior to systemic lymphoid dissemination. Although the two assays displayed similar lymphoid distribution patterns, RT-QuIC analysis identified more positive tissues compared with TSA-IHC. Abbreviations: QuIC, RT-QuIC; IHC, TSA-IHC; Retro LN, retropharyngeal lymph node; VNO, vomeronasal organ; NA, not applicable.
b
Data for each tissue are displayed as the number of animals positive by the detection method out of the total number of animals examined.