FIG 1.

Ccr7 is required for survival and directs DCs and T cells to the draining lymph node during WNV infection. (A) Kaplan-Meier survival analysis of WNV-infected WT (n = 69) and Ccr7^−/− (n = 36) mice. Data shown are pooled from three independent experiments. (B to F) Following forward scatter/side scatter and UV-negative gating for live cells, total cell numbers of mDCs (MHC-II^hi CD11c⁺), CD8⁺ T cells (CD3⁺ CD8⁺), CD4⁺ T cells (CD3⁺ CD4⁺), activated CD8⁺ T cells (CD3⁺ CD8⁺ CD44⁺), and activated CD4⁺ T cells (CD3⁺ CD4⁺ CD44⁺) were assessed by flow cytometry from the popliteal lymph node (LN) from WT and Ccr7^−/− mice. (G) Virus load was quantified in the popliteal LN by quantitative RT-PCR. All data are shown as means ± standard deviations for n = 3 to 8 mice per genotype and time point from two independent experiments. *, P < 0.05; **, P < 0.01. p.i., postinfection; LN, lymph node.