FIG 2.

Leukocytes and proinflammatory cytokines are elevated in the blood of WNV-infected Ccr7^−/− mice. (A to H) Following forward scatter/side scatter and UV-negative gating for live cells, total cell numbers of CD8⁺ T cells (CD3⁺ CD8⁺), CD4⁺ T cells (CD3⁺ CD4⁺), monocytes (Ly6C^hi CD11b⁺), neutrophils (Ly6C^int CD11b⁺ Ly6G⁺), NK cells (NK1.1⁺ CD3⁻), B cells (CD19⁺), activated CD8⁺ T cells (CD3⁺ CD8⁺ CD44⁺), and activated CD4⁺ T cells (CD3⁺ CD4⁺ CD44⁺) were assessed by flow cytometry on days 0, 1, 3, and 5 postinfection from the blood of WT and Ccr7^−/− mice. (I to M) Protein levels of cytokines and chemokines Ccl2, Ccl7, Ccl4, Cxcl9, and IL-10 in the blood of WT and Ccr7^−/− mice at day 0 and at 4 and 8 days postinfection were measured by multiplex ELISA. (N) Virus load was quantified in the blood plasma by quantitative RT-PCR. All data are shown as means ± standard deviations for n = 4 mice per genotype and time point from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.