TABLE 5.
Effect of the CX4C mutation on memory response by CD4 and CD8 splenocytes
| Groupa | % cytokine-producing T cellsb |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4 |
CD8 |
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| Total CD4 |
TMEM |
TCM |
Total CD8 | TMEM | TCM | |||||||||||||||||||
| T-bet | IFN-γ | Gata-3 | IL-4 | T-bet | IFN-γ | Gata-3 | IL-4 | T-bet | IFN-γ | Gata-3 | IL-4 | |||||||||||||
| 1 | 12 ± 0 | 9 ± 5 | 3 ± 1 | 23 ± 2 | 6 ± 2 | 20 ± 4 | 3 ± 0 | 7 ± 1 | 6 ± 1 | 9 ± 3 | 13 ± 2 | 12 ± 2 | 8 ± 1 | 3 ± 0 | 1 ± 0 | 3 ± 0 | 5 ± 1 | 2 ± 1 | 1 ± 0 | 6 ± 1 | 10 ± 2 | 6 ± 0 | 1 ± 0 | 4 ± 0 |
| 2 | 42 ± 4c | 73 ± 3c | 1 ± 0 | 2 ± 0c | 22 ± 2c | 90 ± 3c | 1 ± 1 | 1 ± 0 | 28 ± 2c | 82 ± 3c | 2 ± 0c | 2 ± 0c | 25 ± 5c | 19 ± 2c | 0 ± 0 | 1 ± 0 | 27 ± 1c | 12 ± 1c | 0 ± 0 | 1 ± 0 | 37 ± 3c | 35 ± 4c | 0 ± 0 | 0 ± 0 |
| 3 | 9 ± 3 | 19 ± 3 | 1 ± 0 | 39 ± 3 | 34 ± 3 | 16 ± 2 | 2 ± 0 | 34 ± 6 | 27 ± 2 | 13 ± 3 | 5 ± 1 | 37 ± 7 | 9 ± 2 | 8 ± 1 | 1 ± 0 | 2 ± 0 | 10 ± 1 | 5 ± 1 | 1 ± 0 | 12 ± 0 | 16 ± 2 | 4 ± 1 | 1 ± 0 | 3 ± 0 |
| 4 | 58 ± 9c | 67 ± 5c | 0 ± 0 | 6 ± 0c | 91 ± 4c | 97 ± 3c | 0 ± 0 | 7 ± 1c | 84 ± 3c | 88 ± 3c | 2 ± 0 | 6 ± 0c | 51 ± 1c | 45 ± 1c | 0 ± 0 | 0 ± 0 | 35 ± 1c | 24 ± 2c | 0 ± 0 | 1 ± 0c | 55 ± 0c | 44 ± 7c | 0 ± 0 | 1 ± 0 |
| 5 | 49 ± 5c | 66 ± 5c | 0 ± 0 | 1 ± 0 | 24 ± 1c | 92 ± 1c | 0 ± 0 | 1 ± 0 | 29 ± 1c | 83 ± 1c | 2 ± 1 | 2 ± 0 | 29 ± 3c | 22 ± 4c | 0 ± 0 | 1 ± 0 | 24 ± 2c | 13 ± 0c | 0 ± 0 | 1 ± 0 | 36 ± 2c | 36 ± 3c | 0 ± 0 | 0 ± 0 |
| 6 | 54 ± 1c | 72 ± 3c | 0 ± 0 | 5 ± 0 | 89 ± 3c | 91 ± 2c | 0 ± 0 | 6 ± 0 | 86 ± 1c | 88 ± 1c | 1 ± 0 | 5 ± 0 | 51 ± 0c | 44 ± 2c | 0 ± 0 | 0 ± 0 | 36 ± 2c | 22 ± 1c | 0 ± 0 | 1 ± 1 | 55 ± 4c | 45 ± 3c | 0 ± 0 | 1 ± 0 |
| 7 | 1 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 ± 0 | 0 ± 0 | 0 ± 0 | 1 ± 1 | 1 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 2 ± 0 | 0 ± 0 | 0 ± 0 | 2 ± 1 | 1 ± 0 | 0 ± 0 | 0 ± 0 |
1, wt A2; 2 A2-CX4C; 3, wt r19F; 4, r19F-CX4C; 5, wt A2 plus F(ab′)2 131-2G; 6, wt r19F plus F(ab′)2 131-2G; 7, mock infected.
Frequencies of T-bet and IFN-γ or Gata-3 and IL-4 secretion in CD4, TMEM, or TCM T cells and CD8, TMEM or TCM T cells from peptide stimulated spleenocytes, 75 days p.i. Mice were challenged intranasally with the indicated virus or virus plus G MAb 131-2G (F(ab′)2 administered intraperitoneally 2 days before challenge or mock infected, as described in Materials and Methods. Spleens were harvested 75 days p.i. and processed, and splenocytes were stimulated with an RSV-specific CD4 or CD8 peptide or ovalbumin (Ova) control peptide. (A) CD4 T cell response to RSV-specific CD4 peptide or Ova control peptide. (B) CD8 T cell response to an RSV-specific CD8 peptide or Ova control peptide. The values for Ova stimulation are not shown but were similar to the values for mock-infected mice stimulated with the respective RSV peptide. The values (±SEM) are from 5 mice per group. TMEM, effector memory cells; TCM, central memory cells.
Significant difference (P ≤ 0.001), as determined by one-way ANOVA and post hoc Tukey's HSD test.