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. 2017 Apr 28;91(10):e02048-16. doi: 10.1128/JVI.02048-16

FIG 1.

FIG 1

miR-126-5p is significantly upregulated in primary monocytes during chronic HIV-1 infection and correlated with disease progression. (A) miR-126-5p expression is significantly increased during chronic HIV-1 infection. The expression of miR-126-5p was quantified by qPCR and normalized to the expression of U6 in each sample. (B) The inverse correlation of miR-126-5p expression in monocytes with CD4+ T cell counts. The expression levels of miR-126-5p were used to perform the correlation analysis with CD4+ T cell counts, and a significant negative correlation was observed (rho = −0.53, P = 0.039). (C) The correlation of miR-126-5p expression in monocytes with plasma viral loads. The expression levels of miR-126-5p were used to perform the association with plasma viral loads, and a significant association was observed (rho = 0.58, P = 0.03). (D) An example of a dot plot analysis of three monocyte subsets: CD14++ CD16− (classical) monocytes, CD14+ CD16++ (nonclassical) monocytes, and CD14++ CD16+ (intermediate) monocytes. (E) HIV-1 infection resulted in increased levels of nonclassical and intermediate monocyte subsets, and ART mainly reduces the level of nonclassical monocytes. The percentages of the three monocyte subsets were obtained from HIV− (HIV-1 seronegative; n = 16), ART− (HIV-1 infected, naïve to ART; n = 9), and ART+ (HIV-1 infected, ART treated; n = 12) subjects and averaged into groups; the constituent ratios then were calculated using the averaged data and plotted. (F) miR-126-5p is primarily upregulated in nonclassical monocytes. qPCR was performed on three monocyte subsets (CD14++ CD16−, CD14+ CD16++, and CD14++ CD16+) derived from the ART− (n = 5) and HIV− (n = 5) groups, and the data were averaged by groups. n.s, not significant.