FIG 1.
Analysis of the anti-HIV-1 activity of Ser family members. (A) 293T cells were transfected with an HIV-1 WT or ΔNef proviral vector in the presence of the indicated pCMV6-Ser expression vectors at a 1:1 ratio. After 48 h, virions were collected from the culture supernatants and purified by ultracentrifugation. The purified virions and cells were lysed and analyzed by Western blotting using the indicted antibodies. (B) After virions produced from 293T cells were quantified by p24Gag ELISA, equal amounts of viruses were used to infect the HIV-1 luciferase reporter cell line TZM-b1 to determine viral infectivity. The error bars represent standard deviations (SD) from three experiments. (C) 293T cells were transfected with pCMV6-Ser5 in the presence or absence of WT or ΔNef HIV-1 vector. Viral particles were purified from culture supernatants by ultracentrifugation. Cell and virion lysates were analyzed by Western blotting. The asterisk indicates remnant protein bands from a previous Western blot. The GenBank accession numbers for the Ser proteins are as follows: NM_020755 (human Ser1), NM_178865 (human Ser2), NM_006811 (human Ser3), NM_001258032 (human Ser4*), NM_001174072 (human Ser5), and NM_012032 (murine Ser3).