TABLE 3.
Primers used in the construction of plasmids and RT-qPCR
| Use | Primer name | Sequence (5 ′–3 ′) |
|---|---|---|
| Construction of HBV-miR-3 | HBV-miR-3-S | CGGGGTACCGAGAGCACAACATCAGGATTC |
| HBV-miR-3-AS | GACGAATTCGGACTGAGGCCCCCTCCC | |
| RT-qPCR of HBV-miR-3 | HBV-miR-3/HBV-miR-3-mut RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAACGCCG |
| HBV-miR-3 Forward | TGCGGCTGGATGTGTCTGCGG | |
| RT-qPCR of HBV-miR-3-3p | HBV-miR-3-3p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAGAAGAAC |
| HBV-miR-3 Forward | TGCGGATCTTCTTGTTGGTT | |
| Construction of HBV-miR-3mut | HBV-miR-3-mut-MS | TATCGCAATGCACGTCTGCGGCGTTTTATC |
| HBV-miR-3-mut-MA | GATAAAACGCCGCAGACGTGCATTGCGATA | |
| RT-qPCR of HBV-miR-3mut | HBV-miR-3-mut Forward | TGCGGCAATGCACGTCTGCG |
| RT-qPCR of miR-423 | miR-423 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAGTCTC |
| miR-423 Forward | TGCGGTGAGGGGCAGAGAG | |
| RT-qPCR of miR-16 | miR-16 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACCGCCAAT |
| miR-16 Forward | TGCGGTAGCAGCACGTAAATA | |
| RT-qPCR of U6 snRNA | U6 RT | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACAAAATATGG |
| U6 Forward | TGCGGGTGCTCGCTTCGGCAGC | |
| miRNA Reverse primer | CCAGTGCAGGGTCCGAGGT | |
| Construction of HBV2139 EGFP reporters | EGFP-HBV2139-S | GATCCAGATCCAGCATCCAGGAAGCTTG |
| EGFP-HBV2139-AS | AATTCAAGCTTCCTGGATGCTGGATCTG | |
| EGFP-HBV2139-MS | GATCCAGATCCAGAGAGTCGGAAGCTTG | |
| EGFP-HBV2139-MA | AATTCAAGCTTCCGACTCTCTGGATCTG | |
| Construction of pHBV-1.3-mut | HBV1.3-mut-S | GGAAGATCCAGCCAGTCGAGACCTAGTAGTCAGT |
| HBV1.3-mut-AS | CTAGGTCTCGACTGGCTGGATCTTCCAAATTAAC | |
| RT-qPCR of β-actin | β-actin-S | CGTGACATTAAGGAGAAGCTG |
| β-actin-AS | CTAGAAGCATTTGCGGTGGAC | |
| Construction of HBV 3.5 kb | HBV3.5kb-S | GCAGGCAAGCTTGTTCATGTCCTACTGTTCAAG |
| HBV-mRNA-Reverse | ATAAGAATGCGGCCGCGCAAAAACGAGAGTAACTCC | |
| Construction of HBV 2.4 kb | HBV2.4kb-S | GCAGGCAAGCTTATAAGAGAGAAACAACACAT |
| HBV-mRNA-Reverse | ATAAGAATGCGGCCGCGCAAAAACGAGAGTAACTCC | |
| Construction of HBV 2.1 kb | HBV2.1kb-S | GCAGGCAAGCTTAGAAACACTCATCCTCAGGC |
| HBV-mRNA-Reverse | ATAAGAATGCGGCCGCGCAAAAACGAGAGTAACTCC | |
| Construction of HBV 0.7 kb | HBV0.7kb-S | GCAGGCAAGCTTCATGGC TGCTAGGCTGTGC |
| HBV-mRNA-Reverse | ATAAGAATGCGGCCGCGCAAAAACGAGAGTAACTCC | |
| RT-qPCR of pgRNA/HBV DNA copies | pgRNA-qRT-S | TCTTGCCTTACTTTTGGAAG |
| pgRNA-qRT-AS | AGTTCTTCTTCTAGGGGACC | |
| RT-qPCR of total RNA | Total RNA-qRT-S | CTCCCCGTCTGTGCCTTCTC |
| Total RNA-qRT-AS | TCGGTCGTTGACATTGCTGA | |
| Construction of shR-Dicer | shR-Dicer-S | GATCCCTCGAAATCTTACGCAAATACTCGAGTATTTGCGTAAGATTTCGAGTTTTTGA |
| shR-Dicer-AS | AGCTTCAAAAACTCGAAATCTTACGCAAATACTCGAGTATTTGCGTAAGATTTCGAGG | |
| Construction of shR-Drosha | shR-Drosha-S | GATCCCCAGATGAGACTGAAGACATCTCGAGATGTCTTCAGTCTCATCTGGTTTTTGA |
| shR-Drosha-AS | AGCTTCAAAAACCAGATGAGACTGAAGACATCTCGAGATGTCTTCAGTCTCATCTGGG | |
| Construction of DNA Pol | DNA pol-S | GCCGGTACCGCCATGCCCCTATCTTATCCACAC |
| DNA pol-AS | ATAAGAATGCGGCCGCTCACGGGGGTCTCCATGCGAC | |
| Construction of DNA Pol-mut | DNA pol-M-S | GCCGGTACCGCCATGCCCTATCTTATCCACAC |
| DNA pol-M-AS | ATAAGAATGCGGCCGCTCACGGGGGTCTCCATGCGAC |