FIG 2.

Schematic representation of the mutagenesis strategy used to introduce stabilizing mutations into SAT2/ZIM/7/83. The predicted mutations of the VP2 and VP3 capsid proteins are indicated in single, triple, or quadruple cassettes. The amino acid residues are numbered independently for each protein. In the mutant names, the first digit indicates the protein (VP1, VP2, or VP3) and the last three digits the amino acid position. Following overlap extension mutagenesis, the stabilizing mutated P1 regions were cloned into the SspI and XmaI sites of pSAT2, a genome-length cDNA clone of SAT2/ZIM/7/83. IRES, internal ribosome entry sequence; UTR, untranslated region.