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. 2017 Apr 1;31(7):648–659. doi: 10.1101/gad.293266.116

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© 2017 Latella et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — Cell cycle transitioning is needed to execute the myogenic program. (A–C) Presenescent fibroblasts at PD35 were infected with pBabe (Ctr) and hTERT (hTR) retroviruses and then kept in culture for an additional five PD. (A) Quantification of the percentage of EdU-positive cells. Myogenin (MYOG) (B) and MCK (C) RNA expression levels in empty (Ctr) and hTERT (hTR) BJs expressing MYOD shifted to DM for 48 h. Representative images of EdU (red) incorporation (D) and Myogenin expression (F) by immunofluorescence in HP BJs expressing the MYOD/Y30F mutant treated with APH in DM for 24 h. Quantification of the percentage of EdU-positive (E) and Myogenin-positive (G) cells. (H) Myogenin RNA expression levels in HP BJs (Ctr) and HP BJs expressing the MYOD/Y30F mutant treated with APH in DM for 24 h. (I) Representative images of Myogenin expression by immunofluorescence in HP IMR90 fibroblasts expressing the MYOD/Y30F mutant treated with APH in DM for 24 h. (J) Quantification of the percentage of Myogenin-positive cells. (K) Myogenin and MyHC RNA expression levels in HP IMR90 fibroblasts (Ctr) and HP IMR90 fibroblasts expressing the MYOD/Y30F mutant treated with APH in DM for 24 h. Bar, 50 µm.