The effects of lncRNA-ATB depletion on osteosarcoma cell proliferation, migration, and invasion. A. lncRNA-ATB expression levels in lncRNA-ATB stably depleted and control U2OS cells. B. Cell proliferation of lncRNA-ATB stably depleted and control U2OS cells were assessed by CCK-8 assays, and the relative numbers of cells to 0 h are shown. C. Cell proliferation of lncRNA-ATB stably depleted and control U2OS cells were assessed by EdU incorporation assays. Scale bars, 100 μm. D. The migrated cell numbers of lncRNA-ATB stably depleted and control U2OS cells per field were counted 48 h after seeding. Scale bars, 100 μm. E. The invaded cell numbers of lncRNA-ATB stably depleted and control U2OS cells per field were counted 48 h after seeding. Scale bars, 100 μm. For all panels, results are present as mean ± SD. **P<0.01, ***P<0.001 by Student’s t-test.