Figure 1.

Knock-in of the NRAS^Q61K into BEAS-2B cells enhances angiogenesis. A. Representative images of BEAS-2B cells plated on the CAM. Scale bar: 1 cm. Qualitative assessment of angiogenesis in the CAM assay. Data are from three independent experiments and are mean ± SD. n = 6, **P < 0.01 compared with wild type cells. B. Representative images of BEAS-2B cells growth in the plugs. Quantitative analysis of hemoglobin levels in BEAS-2B plugs. Data are from three independent experiments and are mean ± SD. n = 6, **P < 0.01 compared with wild type cells. Scale bar: 0.5 cm. C. Photopictographs of a typical experiment showing the tube formation in wild type cells and NRAS^Q61K knock-in cells. NRAS^Q61K increased the capillary lengths of BEAS-2B cells (Scale bar represents 50 μm). Data are from three independent experiments and are mean ± SD. n = 3, **P < 0.01 compared with wild type cells.