Figure 7.

Trametinib treatment down-regulates the expression of proangiogenic factors. A. Expression of proangiogenic factors was evaluated by real-time PCR. H1299 and H2087 cells were treated for 24 h with trametinib. Data are expressed as relative quantity (RQ) of trametinib compared with vehicle-treated samples. Bars show mean ± SD of triplicate measurements. B. Quantification of secreted VEGFA in cells supernatant by ELISA after 24 h of the indicated treatments. Data are expressed in pg/mL and bars represent mean ± SD of duplicate observations. C. Biochemical analysis of VEGFA in H1299 and H2087 cells. Protein loading was normalized by GAPDH. D. VEGFA was measured in H1299 and H2087 cell after trametinib treated in the presence of siNRAS^Q61K. GAPDH expression was used as a loading control.