Figure 5.

N-Ras reintroduction reverses the inhibitory effect of miR-1301-3p. A. The rescue experiment was performed by introducing pcDNA3.1-N-Ras or pcDNA3.1 in the presence or absence of ectopic miR-1301-3p or miR-ctrl expression in U87 and U251 cells. Western blot analysis of N-Ras, MEK, p-MEK, ERK1/2 and p-ERK1/2 in the indicated cells. β-Actin was used as the loading control. B-D. Cell viability of glioma cells transfected with pcDNA3.1-N-Ras and miR-1301-3p separately or together was detected using the CCK-8 and colony formation assays. E-H. Cell proliferative potential was evaluated using the EDU assay 48 h after co-transfection. I-L. Cell cycle distribution of glioma cells was measured using flow cytometry.