Figure 5.

Effect of IL1B on ABCC1 (A), ABCC9 (B), SLCO4C1 (C), and SLCO5A1 (D) mRNA levels in endometrial explant cultures. Endometrial explants from gilts on day 12 of the estrous cycle were cultured in DMEM/F-12 with 0, 1, 10, 100 ng/mL IL1B in the presence of both E₂ (50 ng/mL) and P₄ (3 ng/mL), at 37°C for 24 h. All experiments were done in triplicate with endometrium from three gilts for each treatment. Level of mRNA expression determined by real-time RT-PCR analysis is relative to that for ABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs in the control group of endometrial explants (0 ng/mL IL1B) after normalization of transcript amounts to RPL7 mRNA. Data are presented as least squares means with standard errors. IL1B, interleukin-1β; ABCC1, ATP-binding cassette, subfamily C, member 1; ABCC9, ATP-binding cassette, subfamily C, member 9; SLCO4C1, solute carrier organic anion transporter family, member 4C1; SLCO5A1, solute carrier organic anion transporter family, member 5A1; DMEM/F-12, Dulbecco’s modified Eagle medium/F-12; RT-PCR, reverse-transcription polymerase chain reaction; RPL7, ribosomal protein L7.