Figure 2.

Knockout of chicken myostatin (MSTN) gene and identification of mutant genotype in the targeted sites. (A) Fluorescence-activated cell sorting (FACS) of GFP-positive cells after co-transfection of the Cas9-D10A nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs). (B) Phenotypic comparison between regular and MSTN knockout DF1 cells. (C) Mutant genotypes of the targeted MSTN gene induced by the nickase. (D) Western blotting of chicken MSTN in regular and MSTN knockout DF1 cells.