A: Schematic diagram representing the study outline and treatment groups. Wistar rats were subjected to 3 h MCAO using the suture model followed by reperfusion for 21 h. Treatments were administered i.p. at reperfusion in separate syringes. Animals were assigned to one of four treatment groups in a 2X2 study design as outlined in the table.
B: Representative TTC stained brain sections and quantification of infarct size from the four treatment groups. C21 treatment reduced infarct size at 24 h after 3 h MCAO. Co-treatment with anti-IL-10 neutralizing antibody abrogated the neuroprotective effect of C21 treatment. N=7–9 per group. *P<0.05 vs other groups (Two-way ANOVA).