Skip to main content
. Author manuscript; available in PMC: 2018 Mar 15.
Published in final edited form as: Eur J Pharmacol. 2017 Feb 10;799:128–134. doi: 10.1016/j.ejphar.2017.02.016

Fig 2. IL-10 neutralization does not prevent the C21 mediated functional outcome improvement.

Fig 2

Fig 2

A & B: C21 treatment improved Bederson and beam walk scores compared to saline control group, while IL-10 neutralization partially blocked this functional improvement. N=7–9 per group. *P<0.05 vs Sal+IgG and Sal+IL-10_Ab (Two-way ANOVA).

C: Measuring the pro-inflammatory cytokine, TNF-α, using Western blotting showed a band at 52 kDa corresponding to the homotrimeric secreted protein and a band at 26 kDa corresponding to the membrane bound form. Both bands showed similar trend. Stroke lead to an increase in the pro-inflammatory cytokine, TNF-α, in the ischemic hemisphere compared to the sham group. C21 ablated the increase in TNF-α in ischemic hemisphere compared to saline and this was reversed by Anti-IL-10. *P<0.05 vs Sal+IgG and C21+IL-10_Ab. #P<0.05 vs C21+IL-10_Ab group. $P<0.05 vs other groups. n=5–7 per group