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. Author manuscript; available in PMC: 2018 Mar 15.

Published in final edited form as: Eur J Pharmacol. 2017 Feb 10;799:128–134. doi: 10.1016/j.ejphar.2017.02.016

Fig 3 — A: Immunostaining of the primary neurons culture for the neuronal marker MAP2 at day 10 in vitro (DIV 10).

B: Time dependent increased cell death with OGD. Lactate dehydrogenase (LDH) release into the supernatant increased in a time dependent manner after 3, 6, and 9 h of OGD. N=6. One-way ANOVA: *P<0.05 vs control and 3 h OGD, ^#P<0.05 vs 6 h OGD

C: C21 in different concentrations increased cell survival after 6 h OGD as measured by MTT assay. N=3–4. *P<0.05 vs OGD control

Fig 3 — A: Immunostaining of the primary neurons culture for the neuronal marker MAP2 at day 10 in vitro (DIV 10).

B: Time dependent increased cell death with OGD. Lactate dehydrogenase (LDH) release into the supernatant increased in a time dependent manner after 3, 6, and 9 h of OGD. N=6. One-way ANOVA: *P<0.05 vs control and 3 h OGD, ^#P<0.05 vs 6 h OGD

C: C21 in different concentrations increased cell survival after 6 h OGD as measured by MTT assay. N=3–4. *P<0.05 vs OGD control