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. 2017 Apr 15;9(4):1543–1560.

Figure 1.

Figure 1

Flow cytometric quantification of adipose-derived mesenchymal stem cells (ADMSCs), morphological features of ADMSCs and exosomes. A: Flow cytometric identification of ADMSCs through quantification of the expression of surface markers including CD90, CD105, CD29, and CD217 as well as marker of hematopoietic stem cell, CD45, prior to cell culture (i.e., day 0). B: Flow cytometric analysis of markers of ADMSCs, including CD90, CD105, CD29, and CD217 as well as hematopoietic stem cell marker CD45 after cell culture (i.e., day 14). C: Spindle-shaped cells on light microscopy (100x and 200x, respectively) typical of ADMSC at day 14 after cell culture. D: Statistical analysis of the percentage of stem cell population prior to vs. after cell culture (n = 6). For CD90+ cell, * vs. day 0, P<0.0001; for CD105+ cells, † vs. day 0, P<0.0001; for CD29+ cells, ‡ vs. day 0, P<0.0001; for CD217+ cells, § vs. day 0, P<0.001; for CD45+ cells, ¶ vs. day 0, P<0.0001. E: Transmission electronic microscopy showing the morphological feature of exosomes with diameter ranging from 30-100 nm.