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. 2017 Apr 15;9(4):1543–1560.

Figure 6.

Figure 6

Immunofluorescent (IF) staining for identification of inflammatory cell (CD11+, CD14+) infiltration in liver parenchyma 72 hours after reperfusion. A to E: IF microscopy (400 x) demonstrating CD11+ cells (green) in liver parenchyma. Scale bars in right lower corner represent 20 µm. F: Analytic results of number of CD11+ cells, * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001. G to K: IF microscopy (400 x) identifying CD14+ cells (green) in liver parenchyma. Scale bars in right lower corner represent 20 µm. L: Number of CD14+ cells in different groups, * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 10 for each group). Symbols (*, †, ‡, §, ¶) indicate significant differences (at 0.05 level). SC = sham control; LIR = liver ischemia reperfusion; Ex = exosome; Mel = melatonin.