Table 1.
Primers of cytokines for Real-time PCR
| Gene | GenBank ID | Primer | Annealing Tm (°C) | Product Size (bp) |
|---|---|---|---|---|
| IL-2 | NM_008366.3 | 5’-GCGGCATGTTCTGGATTTGACT-3’ | 52.5 | 136 |
| 5’-CTCATCATCGAATTGGCACTCA-3’ | ||||
| IL-4 | M25892.1 | 5’-TCACAGCAACGAAGAACACCAC-3’ | 54.6 | 155 |
| 5’-GCATCGAAAAGCCCGAAAGAGT-3’ | ||||
| IL-6 | NM_031168.1 | 5’-ATGGCAATTCTGATTGTATG-3’ | 49.8 | 212 |
| 5’-GACTCTGGCTTTGTCTTTCT-3’ | ||||
| IFN-γ | NM_008337.3 | 5’-AACTCAAGTGGCATAGATGTGGAAG-3’ | 54.1 | 256 |
| 5’-TGTTGACCTCAAACTTGGCAATAC-3’ | ||||
| TNF-α | BC117057.1 | 5’-TGAGGTCAATCTGCCCAAGTA-3’ | 55.7 | 268 |
| 5’-AGGTCACTGTCCCAGCATCT-3’ | ||||
| β-actin | NM_007393.3 | 5’-AGAGGGAAATCGTGCGTGAC-3’ | 55.9 | 204 |
| 5’-CACAGGATTCCATACCCAAG-3’ |