Figure 4. Enzymatic activity of TPMTwt (black), TPMT p.Y240C (dark grey) and TPMT p.Y240S (light grey) at 37 °C after 0 h, 0.5 h or 8 h incubation at 37 °C.

The relative enzymatic activity was calculated as a percentage of TPMTwt enzymatic activity at time point 0 h and visualized as % of TPMTwt and mean ±SEM (N = 3).