Table 2. PCR and sequencing primers.
| Target Exon | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| M13 tailed intronic primersa | ||
| V | TGTAAAACGACGGCCAGTCTGCATGTTCTTTGAAACCCTATG | CAGGAAACAGCTATGACCCTGCGTGCTAAATAGGAACCATC |
| VII | TGTAAAACGACGGCCAGTGGGACGCTGCTCATCTTCT | CAGGAAACAGCTATGACCGCCTTACACCCAGGTCTCTG |
| X | TGTAAAACGACGGCCAGTAAGTGTTGGGATTACAGGTG | CAGGAAACAGCTATGACCTCCTCAAAAACATGTCAGTGTG |
| Sequencing exon 2 to 10 | ||
| II | AGCCTGGGGGATAGAGAGAG | GCAAACATTGCATAAAAGCAT |
| III | AGGTTTTCATTTAGTTCATCAAT | TTTTTGATAGAACATTTCTCTATTGT |
| IV | TACCACTGACTGGGTGTGTGTCTGA | CTCAATCCAGAAAGACTTCATACCTGTT |
| V | CCCTCTATTTAGTCATTTGAAAAC | ACTTTTGTGGGGATATGGAT |
| VI | GCCCTCTTTCCTTGACTATT | CACAGGAGGAAGAGAGTGAG |
| VII | TGTTGAAGTACCAGCATGCAC | AAAATTACTTACCATTTGCGATCA |
| VIII | CGAAAGTAACTTCTGGCTTC | GGCAACTGGTAAAAGAAAAA |
| IX | ATGCCACATCATCACCTATT | ACAGCATAAGTCCACAAACTT |
| X | CACCCAGCCAATTTTGAGTA | CACCCAGCCAATTTTGAGTA |
| Pyrosequencing | ||
| X | Biotin-CCCAGCCAATTTTGAGTATT | CAATTCCTCAAAACATGTCA |
| Sequencing primer | ||
| TTTACTTTTCTGTAAGTAGA | ||
aPCR primers with M13 tails in italic.