Table 3.
Differentially expressed proteins identified by MALDI MS-MS
| No. | Protein code | Gene ID | KI/WT | KO/WT | KO/KI | Pathways |
| 1 | PKP1 | 18772 | 1.76 | -1.13 | -1.99 | Focal adhesion, apoptosis |
| 2 | HRC | 15464 | 1.62 | 1.06 | -1.53 | Calcium signaling |
| 4 | PYGB | 53313 | 1.61 | 1.16 | -1.39 | Glucagon signaling, insulin signaling |
| 5 | MSN1 | 17698 | -2.68 | 4.71 | 12.57 | Cell shape, actin-cytoskeleton |
| 6 | VINC | 22330 | -4.18 | 4.96 | 20.64 | Cell-cell adhesion, cell shape, actin cytoskeleton |
| 8 | SYNE11 | 64009 | -3.59 | 5.82 | 20.82 | Nucleus-cytoskeleton connection |
| 14 | ADAM10 | 11487 | 1.71 | -1.18 | -2.02 | Inflammation, amiloidosis |
| 17 | TNPO3 | 320938 | -1.11 | 1.51 | 1.67 | Nucleus-cytoskeleton connection |
| 18 | CAPN8 | 170725 | 2.15 | -1.02 | -2.19 | Inflammation |
| 19 | CGNL1 | 68178 | 1.58 | -1.03 | -1.63 | Focal adhesion |
| 20 | VIM | 22352 | 1.43 | 1.40 | -1.02 | Cell division, fibrosis |
| 23 | MYH6 | 17888 | 1.02 | -3.41 | -3.49 | Sarcomere, actin-cytoskeleton |
| 24 | NRAP | 18175 | -1.03 | 1.79 | 1.84 | Focal adhesion, actin cytoskeleton |
| 28 | ANXA3 | 20480 | 1.23 | -1.80 | -2.23 | Prostaglandin synthesis and regulation |
| 29 | LATS2 | 23805 | -1.58 | -1.51 | 1.04 | Hippo signaling pathway, DNA damage |
| 32 | SPTB1 | 20741 | 1.56 | -1.01 | -1.59 | Actin-cytoskeleton |
| 33 | GCC2 | 11426 | 1.04 | -3.78 | -3.92 | Vesicle-mediated transport, retrograde transport at the trans-Golgi-network |
| 37 | ACADS | 12306 | -2.01 | 1.12 | 2.24 | Mitochondrial fatty acid beta-oxidation |
| 39 | FHL2 | 14200 | 1.05 | -2.25 | -2.37 | Focal adhesion, Wnt, calcineurin signaling |
| 39 | MYOZ2 | 59006 | 1.05 | -2.25 | -2.37 | Cytoskeleton, calcineurin signaling, myofibrillogenesis |
| 47 | FGF9 | 14180 | 2.93 | 5.90 | 2.01 | Fibrosis |
| 53 | DST | 13518 | 2.23 | 1.01 | -2.21 | Focal adhesion, actin cytoskeleton |
| 59 | FEZ2 | 56069 | 1.89 | -1.11 | -2.12 | N/A |
| 59 | CSRP3 | 13009 | 1.89 | -1.11 | -2.12 | Stress sensing, myogenesis |
| 62 | MYOM1 | 319565 | -1.37 | 1.58 | 2.15 | Striated muscle contraction |
| 63 | MYOM2 | 17930 | +++ | Sarcomere | ||
| 65 | EZR/MSN | 17698 | +++ | Cell surface organization, adhesion, microtubule |
1
Genes with statistically significant correlation with that of Mypn in mouse hearts. KI: Knock-in Mypn mouse; KO: Knock-out Mypn mouse; WT: Wild type littermates; -: Proteins downregulated compared to WT; +++: Proteins with differentially phosphorylated proteins in KI vs KO; N/A: Not applicable.