Table 2. List of sea bream betanodavirus isolates included in the phylogenetic analysis.

ID isolate	Year	Age	Fish status	Origin	Clinical signs	GenBank Acc. No.		Genotype	Reference
						RNA1	RNA2
VNNV/S.aurata/Farm1/461-1/Nov2014a	2014	Larvae	Farmed	Farm 1	Present	KY354688	KY354702	RGNNV/SJNNV	This work
VNNV/S.aurata/Farm1/461-10/Nov2014a	2014	Larvae	Farmed	Farm 1	Present	KY354690	KY354703	RGNNV/SJNNV	This work
VNNV/S.aurata/Farm1/461-3/Nov2014a	2014	Larvae	Farmed	Farm 1	Present	KY354689	KY354701	RGNNV/SJNNV	This work
VNNV/S.aurata/Farm1/127-1/Mar2015a	2015	Larvae	Farmed	Farm 1	Present	KY354691	KY354704	RGNNV/SJNNV	This work
VNNV/S.aurata/Farm2/575/Nov2015a	2015	Larvae	Farmed	Farm 2	Present	KY354692	KY354705	RGNNV/SJNNV	This work
VNNV/S.aurata/Farm2/165-6/Mar2016a	2016	Larvae	Farmed	Farm 2	Present	KY354693	KY354706	RGNNV/SJNNV	This work
VNNV/S.aurata/I/586/Nov2005b	2005	n.a.	EFS	Italy	Absent	KY354682	KY354698	RGNNV	This work
VNNV/S.aurata/I/425-10/Sep2008b	2008	Juvenile	Farmed	Italy	Absent	KY354681	KY354694	RGNNV	This work
VNNV/S.aurata/I/69-4/Mar2009b	2009	Larvae	Farmed	Italy	Present	KY354683	KY354695	RGNNV/SJNNV	This work
VNNV/S.aurata/I/179-3/May2009b	2009	Larvae	Farmed	Italy	n.a.	KY354684	KY354696	RGNNV/SJNNV	This work
VNNV/S.aurata/CY/203-3/May2010b	2010	Larvae	Farmed	Cyprus	Present	KY354685	KY354697	RGNNV/SJNNV	This work
VNNV/S.aurata/ES/332-1/Aug2010b	2010	Larvae	Farmed	Spain	Absent	KY354686	KY354699	RGNNV	This work
VNNV/S.aurata/ES/339/Aug2010b	2010	Larvae	Farmed	Spain	Absent	KY354687	KY354700	RGNNV	This work
Sa-I-97c	1997	n.a.	Farmed	Italy	Absent	AM085312	AM085337	SJNNV/RGNNV	Toffolo et al.7
Sa-I-00c	2000	n.a.	Farmed	Italy	Absent	AM085314	AM085338	RGNNV	Toffolo et al.7
SpSa-IAusc156.03c	2003	Larvae	Farmed	Spain	Present	FJ803916	FJ803921	RGNNV/SJNNV	Olveira et al.6
PtSa-IAusc61.05c	2005	Larvae	Farmed	Portugal	Present	FJ803912	FJ803918	RGNNV/SJNNV	Olveira et al.6
PtSa-IAusc74.05c	2005	Larvae	Farmed	Portugal	Present	FJ803913	FJ803919	RGNNV/SJNNV	Olveira et al.6
24.1.2005c	2005	Larvae	Farmed	Portugal	Present	JN189844	JN189916	RGNNV/SJNNV	Panzarin et al.8
37.2.2005c	2005	Larvae	Farmed	Portugal	Present	JN189851	JN189918	RGNNV/SJNNV	Panzarin et al.8
58.1.2005c	2005	Larvae	Farmed	Portugal	Present	JN189855	JN189917	RGNNV/SJNNV	Panzarin et al.8
324.1.2006c	2006	n.a.	Farmed	Spain	Present	JN189848	JN189944	RGNNV	Panzarin et al.8
324.2.2006c	2006	n.a.	Farmed	Spain	Present	JN189820	JN189972	RGNNV	Panzarin et al.8
82.4.2007c	2007	Larvae	Farmed	Italy	Present	JX290516	JX290518	RGNNV/SJNNV	Vendramin et al.18
570.16.2008c	2008	n.a.	Wild	Italy	Absent	JN189811	JN189965	RGNNV	Panzarin et al.8
250.1.2009c	2009	Larvae	Farmed	Cyprus	Present	JN189815	JN189920	RGNNV/SJNNV	Panzarin et al.8
250.2.2009c	2009	Larvae	Farmed	Cyprus	Present	JN189845	JN189925	RGNNV/SJNNV	Panzarin et al.8
292.1.2.2009c	2009	Larvae	Farmed	Greece	Present	JN189831	JN189922	RGNNV/SJNNV	Panzarin et al.8
292.3.4.2009c	2009	Larvae	Farmed	Greece	Present	JN189832	JN189923	RGNNV/SJNNV	Panzarin et al.8

The following information is reported: years of isolation, age of the specimen, fish status, country or farm of origin, presence of clinical signs, GenBank accession numbers for RNA1 and RNA2 sequences and reference.

n.a.: not available.

EFS: extensive farming system.

^aSea bream betanodavirus sequences obtained from diseased specimens from Farm 1 and Farm 2.

^bSea bream betanodavirus sequences obtained from viral isolates stored at IZSVe repository.

^cSea bream betanodavirus sequences retrieved from GenBank.