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Colocalization of DNA with several endocytic markers. Transferrin (TF) highlights the involvement of clathrin-mediated endocytosis, cholera toxin B (CTB) the participation of caveolin/raft-mediated endocytosis, and 70 kDa dextran the contribution of fluid-phase endocytosis. DNA was electrotransferred into CHO cells via the application of 10 electric pulses of 5 ms at 1 Hz and 0.4 kV/cm. Images were taken sequentially using wide-field microscopy. Scale bar: 5 µm. From [251].
