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. 2016 Apr;16(2):98–129. doi: 10.2174/1566523216666160331130040

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© 2016 Bentham Science Publishers

This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (15) — Dual-color SPT of DNA aggregates and endosomal proteins in CHO cells after electroporation. CHO cells separately expressing EGFP-Rab5, Rab11, Rab9 and Lamp1 plasmid constructs were electroporated in the presence of Cy5-labeled DNA. Using quantitative colocalization analysis, the respective movements of the objects are investigated. Correlated trajectories are highlighted in orange (DNA) and light purple (EGFP-markers), colocalized trajectories are drawn in blue (DNA) and pink (EGFP-markers) and the non-correlated and non-colocalized trajectories are in red (DNA) and green (EGFP-markers). (a) DNA in early endosomes (Rab5), (b) DNA in recycling endosomes (Rab11), (c) DNA in late endosomes (Rab9), and (d) DNA in lysosomes (Lamp1). Scale bar: 5 µm. From [251].