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. 2017 Mar 17;49(5):435–443. doi: 10.1093/abbs/gmx020

Figure 1.

Figure 1.

Endogenous expression of CC16 in RAW264.7 cells and effects of rCC16 protein on RAW264.7 cell viability (A,B) The endogenous CC16 expression in RAW264.7 cells was detected by RT-PCR (A) and western blot analysis (B). Lane M: DNA marker; Lane 1: positive control (mouse lung sample); Lane 2: RAW264.7 cells sample. β-Actin was used as an internal control. Each assay was repeated three times with similar results. (C,D) RAW264.7 cells were treated with 1.0, 2.5, or 5.0 μg/ml rCC16 or the same volume of PBS for 1–5 days, and cell proliferation and cell viability were determined by CCK-8 assays (C) and Trypan Blue staining (D), respectively. The data are presented as the mean ± SD of three independent experiments. *P < 0.05 relative to vesicle controls. RT-PCR, reverse transcriptase-polymerase chain reaction, DNA, deoxyribonucleic acid.