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. 2017 Mar 17;49(5):435–443. doi: 10.1093/abbs/gmx020

Figure 3.

Figure 3.

rCC16 inhibits the LPS-induced activation and DNA binding of NF-kB but not AP-1 transcription factors in RAW264.7 cells (A–D) Luciferase reporter assay (A, B) and EMSA (C, D) showed the suppression of the transcriptional activity (A) and DNA binding (C) of an NF-κB-responding promoter construct by rCC16 in RAW264.7 cells stimulated with 0.1 μg/ml LPS for 24 h (luciferase reporter assay) or 1 h (EMSA assay). rCC16 had no effect on AP-1 transcriptional activity (B) or DNA binding (D) under the same conditions. Each assay was repeated three times with similar results. The data are presented as the mean ± SD of three independent experiments. **P < 0.01 indicates significant differences between groups as shown. LPS, lipopolysaccharide; AP-1, activator protein; NF-κB, nuclear factor; DNA, deoxyribonucleic acid.