Figure 5.

rCC16 inhibits the LPS-induced phosphorylation of IκBα and p38 MAPK in RAW264.7 cells RAW264.7 cells were treated with rCC16 at 2.0 μg/ml for 2 h and then exposed to LPS exposure at 0.1 μg/ml for another 1 h. The total proteins were extracted, and the levels of phosphorylated IκBα (A) and p38 MAPK (B) were detected by western blot analysis. The degradation of IκBα was detected by western blot analysis (A). One representative of three independent experiments is shown. β-Actin was used as an internal loading control. LPS, lipopolysaccharide.