Figure 6.

Effect of rCC16 on the nuclear translocation of c-Jun, phosphorylation of JNK and c-Jun in LPS-stimulated RAW264.7 cells RAW264.7 cells were treated with rCC16 at 2.0 μg/ml for 2 h and then exposed to LPS exposure at 0.1 μg/ml for another 1 h. (A) The total proteins were extracted, and the levels of phosphorylated JNK, c-Jun, and total JNK, c-Jun were detected by western blot analysis. (B) Subcellular distributions of c-Jun were determined by western blot analysis in nuclear and cytoplasmic preparations. One representative of three independent experiments is shown. β-Actin and lamin B were used as cytosol-fraction or total cell extract and nucleus-fractions internal loading controls, respectively. LPS, lipopolysaccharide; JNK, c-Jun N-terminal kinases .