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. 2017 May 1;15:91. doi: 10.1186/s12967-017-1195-7

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Dilution effect by not-HER2 amplified cells was analyzed in serial dilutions of HER2-positive SKBR3 cell line with HER2-negative MCF10A cell line. Columns represent the mean of three independent experiment performed by Q-PCR (a) and qRT-PCR (b). Copy number and gene expression were measured according to the 2^−[ΔΔCt] algorithm. SKBR3 is a mammary carcinoma cell line with a medium–high level of HER2 amplification. HER2 human epidermal growth factor receptor 2, Q-PCR quantitative polymerase-chain-reaction, qRT-PCR quantitative reverse transcriptase polymerase-chain-reaction