Figure 6.

Effect of ITC on cellular components of E. coli. Transmission electron micrographs of untreated control, treated with ITC at 30 and 300 μg ml⁻¹, and H₂O₂ at 300 μg ml⁻¹ for 2 h, and visualized at 10,000× (A) and at 20,000× magnifications (B). Cell counts (log₁₀ cfu ml⁻¹) of viable E. coli cells from the same samples prior to preparation for TEM (C). Bacterial DNA damage detection by agarose gel electrophoresis (D). Samples 1, 2, 3, and 4 E. coli cells were treated 30 min with ITC at 30 and 300 μg ml⁻¹, H₂O₂ at 30 and 300 μg ml⁻¹, respectively. gDNA was extracted and gel electrophoresis was performed for examination of DNA cleavage (DNA smear). C is gDNA from untreated cells, M is a 1 kb DNA marker.