Table 1.
Gene | Primers | Nucleotide sequences 5′-3′ | Length (bp) | Temp. (°C) |
---|---|---|---|---|
ERK | Forward | AAAAGGGGGAGGGGACGTAA | 87 | 60.5 |
Reverse | CCACTCACCTGTTTCCTGTCT | 59.6 | ||
JNK | Forward | GCCTGGACTGTGACGTCTATTT | 195 | 60.2 |
Reverse | AGCATTCCCTACAAAGTGCC | 58.7 | ||
RhoA | Forward | TGTTTTTCCATCGACAGCCCT | 171 | 60.2 |
Reverse | TACCGGCTCCTGCTTCATTT | 59.4 | ||
ROCK1 | Forward | ACCTTCTGGCTTTGTCCGT | 159 | 59.2 |
Reverse | CCTGGTGATCAGGTAGCAT | 57.4 | ||
ROCK2 | Forward | TCCCTTCCCTGTGGTGACTT | 88 | 60.4 |
Reverse | GGGAAAGGAGCTGGTTCAAGA | 59.9 | ||
MLC | Forward | TCGACGCCATGATGAAGGAA | 200 | 59.5 |
Reverse | CTGGGAAAATCGGTCGCACT | 60.7 | ||
GAPDH | Forward | ATTCCATCCCAGACCCCATAAC | 81 | 59.5 |
Reverse | GCAGCGAACTTTATTGATGGTAT | 57.6 |