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. 2017 Apr 18;2017:3903898. doi: 10.1155/2017/3903898

Table 1.

Nucleotide sequences of primers used in real-time PCR.

Gene Primers Nucleotide sequences 5′-3′ Length (bp) Temp. (°C)
ERK Forward AAAAGGGGGAGGGGACGTAA 87 60.5
Reverse CCACTCACCTGTTTCCTGTCT 59.6
JNK Forward GCCTGGACTGTGACGTCTATTT 195 60.2
Reverse AGCATTCCCTACAAAGTGCC 58.7
RhoA Forward TGTTTTTCCATCGACAGCCCT 171 60.2
Reverse TACCGGCTCCTGCTTCATTT 59.4
ROCK1 Forward ACCTTCTGGCTTTGTCCGT 159 59.2
Reverse CCTGGTGATCAGGTAGCAT 57.4
ROCK2 Forward TCCCTTCCCTGTGGTGACTT 88 60.4
Reverse GGGAAAGGAGCTGGTTCAAGA 59.9
MLC Forward TCGACGCCATGATGAAGGAA 200 59.5
Reverse CTGGGAAAATCGGTCGCACT 60.7
GAPDH Forward ATTCCATCCCAGACCCCATAAC 81 59.5
Reverse GCAGCGAACTTTATTGATGGTAT 57.6