. 2016 Sep 29;13(6):22–26. doi: 10.21010/ajtcam.v13i6.5

Table 1.

Nitric oxide inhibitory activity and cytotoxicity of S. spinosa leaf extracts and fractions based on IC₅₀ and LC₅₀ values.

Samples	LC₅₀, μg/mL)	IC₅₀ μg/mL)
Extracts
EA	27.36±0.85¹	325.14±1.28^a
EM	100. 91±0.03^c	198.35±1.40^b
ED/M	31.41±0.34^h	115.62±0.98^b
EAl	92. 62±0.27^e	300.23±1.20^a

Fractions
WF	50.00±0.08^f	88.43±0.22^c
nBF	98.75±0.00^d	225.11±0.73^a,^b
EtAF	162.42±0.00^a	> 750^d
CF	37.38±0.08^g	96.72±0.10^c
HF	143.21±0.00^b	300.51±2.00^a

Control
Quercitin	nd	36.17 ± 1.78^c
Doxorubicin	4.59±0.01^j	Nd

EA: extract of acetone, EM: extract, of methanol ED/M: Extract of Dichloromethane/methanol, EAl: Extract of Alkaloids, HF: nhexane fraction, CF: Chloroform fraction, EtAF: Ethyl acetate fraction, nBF: n-Butanol fraction, WF: Water fraction. Values with different letters are significantly different at p < 0.05. nd: not determined.