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TLC done with the mentioned solvent combinations at different ratios with the results as noted.
| Solvent combination taken in the ratio of 1:9 to 9:1 | Observation |
|---|---|
| Methanol : hexane | No separation |
| Methanol: petroleum ether | Smear formation |
| Chloroform : hexane | No separation |
| Chloroform: pet. ether | No separation |
| Methanol : dichloromethane | At the ratio of 6:4, two bands were obtained |
